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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
7
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pubmed:dateCreated |
1981-1-16
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pubmed:databankReference |
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pubmed:abstractText |
Poly(A)-containing RNA from the bovine anterior pituitary has been used as a template for the enzymatic synthesis of double-stranded cDNA. The resulting double-stranded cDNA was inserted into the Pst I site of pBR322 with the oligo(dG)-oligo(dC) tailing technique and subsequently cloned in E. coli chi 1776. Clones containing sequences complementary to prolactin mRNA were identified by colony hybridization with partially purified prolactin cDNA. A 250 base pair sequence from one prolactin positive clone was extensively characterized and shown to contain the coding information for amino acids 119-192 of authentic bovine prolactin. The recombinant DNA from this clone was covalently attached to diazotized aminocellulose and used to purify prolactin mRNA from a mixture of mRNAs.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7001369-1105573,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7001369-1195397,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7001369-1262349,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7001369-13267987,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7001369-169893,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7001369-265521,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7001369-266704,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7001369-266726,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7001369-267930,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7001369-270674,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7001369-322279,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7001369-344137,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7001369-353736,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7001369-358147,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7001369-358198,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7001369-369596,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7001369-375200,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7001369-418408,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7001369-4342968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7001369-435463,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7001369-436750,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7001369-4858491,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7001369-4868544,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7001369-4919547,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7001369-4966087,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7001369-631324,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7001369-70785,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7001369-762150,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7001369-821486,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7001369-911801
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0305-1048
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
11
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pubmed:volume |
8
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1561-73
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:7001369-Animals,
pubmed-meshheading:7001369-Cattle,
pubmed-meshheading:7001369-Cloning, Molecular,
pubmed-meshheading:7001369-DNA, Single-Stranded,
pubmed-meshheading:7001369-Escherichia coli,
pubmed-meshheading:7001369-Genes,
pubmed-meshheading:7001369-Nucleic Acid Hybridization,
pubmed-meshheading:7001369-Pituitary Gland, Anterior,
pubmed-meshheading:7001369-Plasmids,
pubmed-meshheading:7001369-Prolactin,
pubmed-meshheading:7001369-RNA, Messenger,
pubmed-meshheading:7001369-Transformation, Genetic
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pubmed:year |
1980
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pubmed:articleTitle |
Construction and characterization of a cDNA clone containing a portion of the bovine prolactin sequence.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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