Linked Life Data

6997641

Source:http://linkedlifedata.com/resource/pubmed/id/6997641

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1980-11-20
pubmed:abstractText
A technique for using routine rapid Golgi impregnation procedures on very thin freshly fixed slices (less than 0.5 mm) of brain tissue is described. The technique was particularly successful with hippocampal slices that were maintained and stimulated in vitro prior to fixation. Thin tissue slices were surrounded by thicker sections of tissue to form a 5 mm thick bundle. The tissue bundle was then processed by a rapid Golgi procedure, 5 days each in chromate osmium and silver nitrate solutions. At the end of this time the thin tissue slices were unwrapped from their thicker protecting tissue sections, embedded in celloidin, cut at 60-100 micrometer thickness on a sliding microtome and mounted in permount under cover glass. Qualitative light microscopic analysis of the rapid Golgi impregnated slices revealed fully impregnated cell bodies, dendrites, dentritic spines, axons and axonal varicosities with minimal background artifact. In contrast, unprotected thin tissue slices showed only a dense black artifact without cells or processes.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0165-0270
pubmed:author
pubmed:issnType
Print
pubmed:volume
2
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
363-71
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1980
pubmed:articleTitle
A Golgi impregnation technique for thin brain slices maintained in vitro.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.