Linked Life Data

6997286

Source:http://linkedlifedata.com/resource/pubmed/id/6997286

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1980-11-25
pubmed:abstractText
The time-course of insulin binding to isolated adipocytes pretreated with chloroquine (0.1 mM) showed that no steady state condition was reached and that the binding kept increasing with the time of incubation up to three hours. Sixty-one percent of the bound labeled insulin could not be dissociated from these cells by 100 microgram/ml of unlabeled insulin, whereas 86% dissociated from the control cells. Chromatography revealed that the bound intact insulin, which had the ability to bind to insulin receptors of adipocytes, was increased and that degraded insulin decreased in the chloroquine treated cells. However, these cells showed normal insulin stimulation of 2-deoxy-glucose uptake. The data suggest that insulin is internalized in adipocytes after binding to insulin receptors and that insulin is degraded at the site, probably lysosomes, where chloroquine inhibits this degradation process.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0021-924X
pubmed:author
pubmed:issnType
Print
pubmed:volume
88
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
39-44
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
1980
pubmed:articleTitle
Receptor mediated insulin degradation decreased by chloroquine in isolated rat adipocytes.
pubmed:publicationType
Journal Article