Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1983-12-20
pubmed:abstractText
We performed radioimmunoprecipitation assays in which iodinated preparations of A/Port Chalmers/73 (A/PC/73) hemagglutinin were used as the test antigens and high concentrations of unlabeled A/Hong Kong/68 viral protein were used to inhibit the binding of cross-reactive antibodies to quantitate strain-specific antibody responses in postvaccination sera. Strain-specific antibodies comprised 8 to 48% (mean, 20%) of the total A/PC/73 antigen-binding capacity of the sera tested. Competition radioimmunoprecipitation assays in which disrupted preparations of purified whole virus representative of several of the H3N2 variants were used indicated that the A/PC/73 strain-specific antibody that was present after adsorption of serum by A/Hong Kong/68 antigen was capable of reacting with A/England/72 and A/Victoria/75 hemagglutinins, but generally with lower avidity than with A/PC/73 hemagglutinin. A comparison of the A/PC/73 antibody titers measured by radioimmunoprecipitation and hemagglutination inhibition tests before and after adsorption with A/Hong Kong/68 whole virus suggested that cross-reactive and strain-specific antibodies were comparable in efficiency of inhibiting viral hemagglutination. These data indicated that vaccines containing later variants within a subtype could induce antihemagglutinin antibodies of restricted specificity, but that these antibodies may not be directed against unique antigenic determinant(s).
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/6985298-13910995, http://linkedlifedata.com/resource/pubmed/commentcorrection/6985298-14200375, http://linkedlifedata.com/resource/pubmed/commentcorrection/6985298-14907713, http://linkedlifedata.com/resource/pubmed/commentcorrection/6985298-4133135, http://linkedlifedata.com/resource/pubmed/commentcorrection/6985298-564916, http://linkedlifedata.com/resource/pubmed/commentcorrection/6985298-6156537, http://linkedlifedata.com/resource/pubmed/commentcorrection/6985298-6162101, http://linkedlifedata.com/resource/pubmed/commentcorrection/6985298-6163993, http://linkedlifedata.com/resource/pubmed/commentcorrection/6985298-6164247, http://linkedlifedata.com/resource/pubmed/commentcorrection/6985298-81808, http://linkedlifedata.com/resource/pubmed/commentcorrection/6985298-88484, http://linkedlifedata.com/resource/pubmed/commentcorrection/6985298-88804, http://linkedlifedata.com/resource/pubmed/commentcorrection/6985298-90369
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0095-1137
pubmed:author
pubmed:issnType
Print
pubmed:volume
15
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
87-92
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1982
pubmed:articleTitle
Characterization of human serum strain-specific antihemagglutinin antibody to A/Port Chalmers/73 (H3N2) influenza virus by radioimmunoprecipitation assays.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.