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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1983-12-20
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pubmed:abstractText |
We performed radioimmunoprecipitation assays in which iodinated preparations of A/Port Chalmers/73 (A/PC/73) hemagglutinin were used as the test antigens and high concentrations of unlabeled A/Hong Kong/68 viral protein were used to inhibit the binding of cross-reactive antibodies to quantitate strain-specific antibody responses in postvaccination sera. Strain-specific antibodies comprised 8 to 48% (mean, 20%) of the total A/PC/73 antigen-binding capacity of the sera tested. Competition radioimmunoprecipitation assays in which disrupted preparations of purified whole virus representative of several of the H3N2 variants were used indicated that the A/PC/73 strain-specific antibody that was present after adsorption of serum by A/Hong Kong/68 antigen was capable of reacting with A/England/72 and A/Victoria/75 hemagglutinins, but generally with lower avidity than with A/PC/73 hemagglutinin. A comparison of the A/PC/73 antibody titers measured by radioimmunoprecipitation and hemagglutination inhibition tests before and after adsorption with A/Hong Kong/68 whole virus suggested that cross-reactive and strain-specific antibodies were comparable in efficiency of inhibiting viral hemagglutination. These data indicated that vaccines containing later variants within a subtype could induce antihemagglutinin antibodies of restricted specificity, but that these antibodies may not be directed against unique antigenic determinant(s).
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pubmed:grant | |
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6985298-13910995,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6985298-14200375,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6985298-14907713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6985298-4133135,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6985298-564916,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6985298-6156537,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6985298-6162101,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6985298-6163993,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6985298-6164247,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6985298-81808,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6985298-88484,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6985298-88804,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6985298-90369
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0095-1137
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
15
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
87-92
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:6985298-Antibodies, Anti-Idiotypic,
pubmed-meshheading:6985298-Cross Reactions,
pubmed-meshheading:6985298-Hemagglutination Inhibition Tests,
pubmed-meshheading:6985298-Hemagglutinins,
pubmed-meshheading:6985298-Humans,
pubmed-meshheading:6985298-Influenza A Virus, H3N2 Subtype,
pubmed-meshheading:6985298-Influenza A virus,
pubmed-meshheading:6985298-Radioimmunoassay,
pubmed-meshheading:6985298-Species Specificity
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pubmed:year |
1982
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pubmed:articleTitle |
Characterization of human serum strain-specific antihemagglutinin antibody to A/Port Chalmers/73 (H3N2) influenza virus by radioimmunoprecipitation assays.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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