Linked Life Data

6983452

Source:http://linkedlifedata.com/resource/pubmed/id/6983452

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
1983-2-25
pubmed:abstractText
The expression of aminopeptidase, a surface-membrane-bound enzyme, on macrophages formed in liquid cultures of hemopoietic progenitor cells was studied over a period of 20 days. The cultures were stimulated by two biochemically distinct types of colony-stimulating factor (CSF) derived from mouse-lung-conditioned medium (MLCM) and L-cell-conditioned medium (LCCM), respectively. The enzyme content of single cells was determined microphotometrically after staining with Fast Blue B salt and leucine 4-methoxy-2-naphthylamide. In LCCM-stimulated cultures the number of cells expressing aminopeptidase, the enzyme content per cell and the enzyme concentration increased markedly from day 10 onward, while macrophages from MLCM-stimulated cultures only showed borderline yet significantly positive aminopeptidase levels. Maximum enzyme concentrations were found earlier than maximum enzyme content indicating an early local increase in the aminopeptidase concentration on the membrane and subsequently a more uniform distribution over the cell surface. The two types of CSF differ not only in their effect on macrophage production but also in their influence on the expression of the surface enzyme aminopeptidase on these cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0301-472X
pubmed:author
pubmed:issnType
Print
pubmed:volume
10
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
789-97
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1982
pubmed:articleTitle
The effect of two different types of colony-stimulating factor on the expression of aminopeptidase on marrow-derived murine macrophages.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't