Linked Life Data

6983152

Source:http://linkedlifedata.com/resource/pubmed/id/6983152

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4-5
pubmed:dateCreated
1983-1-27
pubmed:abstractText
In vitro growth of erythroid progenitors (BFU-e and CFU-e) from mouse bone marrow (BM) can be influenced by the addition of graded doses of thymocytes (THY). At low THY:BM ratios (1:50), inhibition of erythroid growth occurred, whereas at high THY:BM ratios (20:1), enhancement of erythroid growth was observed. Experiments were designed to examine the sensitivity of the thymocytes to gamma radiation or treatment in vitro with an alkylating agent, 4-hydroperoxycyclophosphamide (4HC), to determine if two subpopulations with different physical characteristics were responsible for these observations. We reasoned that if there were selective effects of either radiation or drug treatment on enhancement or inhibition, this would provide evidence for separate populations which regulated erythroid cell growth by cell-cell interaction. At the small doses of drug or X-ray studies (i.e., 10 microM 4HC or 3.0 Gys), elimination of the inhibitory effect of low THY:BM ratios resulted. Enhancement of erythroid growth was unaffected by drug or radiation until 80 microM 4HC or 30.0 Gys were employed. Fresh putative inhibitory thymocytes (low numbers) added to either 4HC or radiation-treated inhibitory cells restored the suppressor effect on erythroid growth. The helper effect, however, was not restored by the addition of fresh thymocytes. We conclude that there are at least two populations which provide (a) an inhibitory influence that is relatively sensitive to radiation and cyclophosphamide in vitro, and (b) an enhancing effect which is relatively insensitive to these treatments.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0250-6793
pubmed:author
pubmed:issnType
Print
pubmed:volume
1
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
269-75
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1982
pubmed:articleTitle
Effect of radiation and 4-hydroperoxycyclophosphamide on thymic regulators of erythropoietic growth.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S.