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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
4
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pubmed:dateCreated |
1982-10-21
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pubmed:abstractText |
To investigate the mechanisms that modulate granulocyte-macrophage colony-stimulating activity (GM-CSA) and burst promoting activity (BPA) elaboration, we studied human peripheral blood-derived monocyte-macrophage (M0) and T-lymphocyte (TL) interaction. Coincubation of live M0 with autologous TL at a 1:3 ratio in the presence of 1% phytohemagglutinin synergistically increased GM-CSA (6 of 6 experiments) and BPA (4 of 6 experiments) (P less than 0.002). Prior treatment of M0 with cycloheximide or actinomycin D significantly (P less than 0.002) diminished this M0's capacity to collaborate with TL. Mitomycin C treatment did not. Live M0 also enhanced TL-derived GM-CSA (P less than 0.002) and BPA (P less than 0.001). This enhancement was again compromised by prior cycloheximide or actinomycin D treatment, but not by mitomycin C treatment. Further experiments in which we blocked DR antigen on M0 membrane with monoclonal anti-DR antibodies suggested that M0 required their membrane DR antigen to collaborate with TL in elaborating GM-CSA and BPA.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Colony-Stimulating Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Culture Media,
http://linkedlifedata.com/resource/pubmed/chemical/HLA-DR Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/Histocompatibility Antigens Class II
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0361-8609
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
12
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
403-10
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:6981350-Bone Marrow Cells,
pubmed-meshheading:6981350-Cell Communication,
pubmed-meshheading:6981350-Colony-Forming Units Assay,
pubmed-meshheading:6981350-Colony-Stimulating Factors,
pubmed-meshheading:6981350-Culture Media,
pubmed-meshheading:6981350-Erythropoiesis,
pubmed-meshheading:6981350-Granulocytes,
pubmed-meshheading:6981350-HLA-DR Antigens,
pubmed-meshheading:6981350-Histocompatibility Antigens Class II,
pubmed-meshheading:6981350-Humans,
pubmed-meshheading:6981350-Macrophages,
pubmed-meshheading:6981350-Monocytes,
pubmed-meshheading:6981350-T-Lymphocytes
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pubmed:year |
1982
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pubmed:articleTitle |
DR antigen positive monocyte-macrophages control granulocyte-macrophage colony-stimulating activity and burst promoting activity elaboration in man.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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