Linked Life Data

6980125

Source:http://linkedlifedata.com/resource/pubmed/id/6980125

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1982-9-24
pubmed:abstractText
Several new aspects of the chemistry, genetics and cellular distribution of the murine Qa-2 alloantigen were apparent in an analysis of this antigen using monoclonal antibodies recognizing a Qa-2-like antigen called Qa-m2. Immunoprecipitation and sodium dodecyl sulfate polyacrylamide gel electrophoresis analysis identified the Qa-m2 alloantigen as a two-chain structure composed of a 39 000 dalton heavy chain and a 12 000 light chain which is probably beta 2-microglobulin; the heavy chain was readily distinguished from that of H-2. In addition, the expression of Qa-m2 alloantigens on the cell surface was found to be controlled by the H-2D gen with H-2Db strains carrying approximately 8-10 times greater amounts of Qa-m2 than strains carrying the H-2Dd or H-2Dq alleles. Finally, the Qa-m2 antigen, found predominantly on peripheral T cells, was present on only 10% of thymus cells. However, subpopulations of B cells (approximately 25% of all B cells) and bone marrow cells (15-20%) were also reactive. The monoclonal anti-Qa-m2 antibodies differed in their reactions from that reported for the conventional anti-Qa-2 sera, which must, therefore, be complex. The monoclonal antibodies may be useful reagents for functional analysis of T and B cell subpopulations.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0014-2980
pubmed:author
pubmed:issnType
Print
pubmed:volume
12
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
374-9
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
1982
pubmed:articleTitle
Description of a Qa-2 like alloantigen (Qa-m2).
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't