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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1978-11-18
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pubmed:abstractText |
1. A high-molecular-weight glycoprotein constitutes over 80% by weight of the total glycoprotein from water-soluble pig colonic mucus. 2. It was isolated from from nucleic acid and non-covalently bound protein by nuclease digestion followed by equilibrium centrifugation in a CsCl gradient. 3. The glycoprotein has the following composition by weight: fucose 10.4%; glucosamine 23.9%; galactosamine 8.3%; sialic acid 9.9%; galactose 20.8%; sulphate 3.0%; protein 13.3%; moisture about 10%. 4. The native glycoprotein has the high mol.wt. of 15 X 10(6). 5. Reduction of the native glycoprotein with 2-mercaptoethanol results in a glycoprotein of mol.wt. 6 X 10(6). 6. Pronase digestion removes 29% of the protein (3% of the glycoprotein) but none of the carbohydrate. 7. The molecular weight of the Pronase-digested glycoprotein is 1.5 X 10(6), which is halved to 0.76 X 10(6) on reduction with 2-mercaptoethanol. 8. The contribution of non-covalent interactions, disulphide bridges and the non-glycosylated peptide core to the quaternary structure of the glycoprotein are discussed and compared with the known structure of pig gastric glycoportein.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/697737-1004543,
http://linkedlifedata.com/resource/pubmed/commentcorrection/697737-13104872,
http://linkedlifedata.com/resource/pubmed/commentcorrection/697737-13293190,
http://linkedlifedata.com/resource/pubmed/commentcorrection/697737-13860975,
http://linkedlifedata.com/resource/pubmed/commentcorrection/697737-14342494,
http://linkedlifedata.com/resource/pubmed/commentcorrection/697737-14468471,
http://linkedlifedata.com/resource/pubmed/commentcorrection/697737-342045,
http://linkedlifedata.com/resource/pubmed/commentcorrection/697737-4161440,
http://linkedlifedata.com/resource/pubmed/commentcorrection/697737-4226469,
http://linkedlifedata.com/resource/pubmed/commentcorrection/697737-4340508,
http://linkedlifedata.com/resource/pubmed/commentcorrection/697737-4463953,
http://linkedlifedata.com/resource/pubmed/commentcorrection/697737-4463954,
http://linkedlifedata.com/resource/pubmed/commentcorrection/697737-4562023,
http://linkedlifedata.com/resource/pubmed/commentcorrection/697737-4747775,
http://linkedlifedata.com/resource/pubmed/commentcorrection/697737-4933894,
http://linkedlifedata.com/resource/pubmed/commentcorrection/697737-5044048,
http://linkedlifedata.com/resource/pubmed/commentcorrection/697737-5113494,
http://linkedlifedata.com/resource/pubmed/commentcorrection/697737-5124389,
http://linkedlifedata.com/resource/pubmed/commentcorrection/697737-5144210,
http://linkedlifedata.com/resource/pubmed/commentcorrection/697737-5338533,
http://linkedlifedata.com/resource/pubmed/commentcorrection/697737-5428049,
http://linkedlifedata.com/resource/pubmed/commentcorrection/697737-5451909,
http://linkedlifedata.com/resource/pubmed/commentcorrection/697737-5495734,
http://linkedlifedata.com/resource/pubmed/commentcorrection/697737-5637714,
http://linkedlifedata.com/resource/pubmed/commentcorrection/697737-5660100,
http://linkedlifedata.com/resource/pubmed/commentcorrection/697737-863903,
http://linkedlifedata.com/resource/pubmed/commentcorrection/697737-869929
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
173
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
569-78
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:697737-Amino Acids,
pubmed-meshheading:697737-Animals,
pubmed-meshheading:697737-Centrifugation, Density Gradient,
pubmed-meshheading:697737-Chemical Phenomena,
pubmed-meshheading:697737-Chemistry,
pubmed-meshheading:697737-Colon,
pubmed-meshheading:697737-Glycoproteins,
pubmed-meshheading:697737-Intestinal Secretions,
pubmed-meshheading:697737-Mercaptoethanol,
pubmed-meshheading:697737-Molecular Weight,
pubmed-meshheading:697737-Mucus,
pubmed-meshheading:697737-Pronase,
pubmed-meshheading:697737-Swine
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pubmed:year |
1978
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pubmed:articleTitle |
The isolation and characterization of the high-molecular-weight glycoprotein from pig colonic mucus.
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pubmed:publicationType |
Journal Article
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