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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1981-8-27
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pubmed:abstractText |
A luminol-enhancement chemiluminescence assay and a radiolabeled uptake assay were developed to assess opsonins for Haemophilus influenzae type b. Opsonins in acute and convalescent sera from 17 children with H. influenzae type b meningitis, along with pooled normal human sera, were evaluated and compared with anti-polyribosephosphate antibody concentrations. Five children had a rise in the chemiluminescence-area under the curve for convalescent compared with acute sera. Patient chemiluminescence--area-under-the-curve values were significantly (P less than 0.05) more likely to exceed 50% of normal human serum values if sera contained greater than or equal to 0.1 microgram of anti-polyribosephosphate antibody per ml. Magnesium ethylene glycol tetraacetic acid chelation and heat inactivation of patient and normal human sera significantly (P less than 0.05) reduced chemiluminescence--area-under-the-curve activity. Thus, complement appears to contribute significantly to the opsonization of H. influenzae type b in sera of children. Two of nine children had increases in opsonins as assayed by 3H-labeled H. influenzae type b uptake. After natural systemic H. influenzae type b infection, young children are unable to respond acutely with an increase in anti-polyribosephosphate antibody or serum opsonic activity.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6972383-1086832,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6972383-1086875,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6972383-1099117,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6972383-215546,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6972383-307539,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6972383-323143,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6972383-335348,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6972383-375175,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6972383-387613,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6972383-4120458,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6972383-4137338,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6972383-4536614,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6972383-4536615,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6972383-4542337,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6972383-4542777,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6972383-6988524,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6972383-70407,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6972383-80382
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0095-1137
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
13
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
532-9
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:6972383-Antibodies, Bacterial,
pubmed-meshheading:6972383-Biological Assay,
pubmed-meshheading:6972383-Child, Preschool,
pubmed-meshheading:6972383-Haemophilus influenzae,
pubmed-meshheading:6972383-Humans,
pubmed-meshheading:6972383-Infant,
pubmed-meshheading:6972383-Luminescent Measurements,
pubmed-meshheading:6972383-Meningitis, Haemophilus,
pubmed-meshheading:6972383-Neutrophils,
pubmed-meshheading:6972383-Opsonin Proteins,
pubmed-meshheading:6972383-Polysaccharides, Bacterial
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pubmed:year |
1981
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pubmed:articleTitle |
Assessment of Haemophilus influenzae type b opsonins by neutrophil chemiluminescence.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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