Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5-6
|
| pubmed:dateCreated |
1981-6-13
|
| pubmed:abstractText |
A highly selected alloreactive T-cell line was developed by repeated restimulation of B10.D2/n lymph-node cells with irradiated C57BL/10Sn (B10) spleen cells in long-term MLC for up to 21/2 years. Continuous growth of the line requires restimulation every 2 to 4 weeks with fresh H-2b stimulator cells. The line proliferates strongly against H-2b but not against H-2d, H-2f, H-2q, H-2r, or H-2s stimulators. Analysis of recombinant mouse strains showed that the proliferative response is directed against I-Ab, but not Kb or Db determinants. During the growth period of the line, strong cross-reactivity with H-2p (B10.P) and weak cross-reactivity with H-2k strains (e.g., CBA/J and B10.BR) was observed. A clone with exquisite specificity for I-Ab, but with no cross-reactivity with H-2p or H-2k was isolated from the line; thus clonal heterogeneity of the line still exists despite the highly selective growth conditions.--The majority of T cells from the line or clone were shown to bind I-Ab but not Kb or Db determinants either spontaneously during restimulation with fresh B10 stimulator cells or via membrane vesicles expressing I-Ab determinants. No killing activity by the line in either specific or nonspecific cytolytic T-cell assays was observed nor was the T 145 glycoprotein, characteristic of killer T cells, detected.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Mar
|
| pubmed:issn |
0093-7711
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
1
|
| pubmed:volume |
12
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
525-40
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:6971252-Animals,
pubmed-meshheading:6971252-Antibody Specificity,
pubmed-meshheading:6971252-Antigens, Surface,
pubmed-meshheading:6971252-Binding Sites,
pubmed-meshheading:6971252-Cell Division,
pubmed-meshheading:6971252-Cells, Cultured,
pubmed-meshheading:6971252-Clone Cells,
pubmed-meshheading:6971252-Kinetics,
pubmed-meshheading:6971252-Lymphocyte Activation,
pubmed-meshheading:6971252-Mice,
pubmed-meshheading:6971252-Mice, Inbred AKR,
pubmed-meshheading:6971252-Mice, Inbred C3H,
pubmed-meshheading:6971252-Mice, Inbred C57BL,
pubmed-meshheading:6971252-Mice, Inbred CBA,
pubmed-meshheading:6971252-Mice, Inbred DBA,
pubmed-meshheading:6971252-Phenotype,
pubmed-meshheading:6971252-Receptors, Antigen, T-Cell,
pubmed-meshheading:6971252-T-Lymphocytes,
pubmed-meshheading:6971252-Time Factors
|
| pubmed:year |
1981
|
| pubmed:articleTitle |
Receptor specificity, functional characteristics, and cell-surface phenotype of a highly selected anti-I-Ab-specific, long-term T-cell line.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|