Linked Life Data

69677

Source:http://linkedlifedata.com/resource/pubmed/id/69677

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1977-9-17
pubmed:abstractText
The spontaneous expression of a type C virus in a diploid strain of human embryonic lung fibroblast-like cells (HEL-12) was examined during serial culture. Virus antigen expression was determined by indirect immunofluorescenc with antisera to disrupted simian sarcoma virus and the 28000 mol. wt. internal antigen of the endogenous cat virus RD-114. Virus production was examined by reverse transcriptase assays of culture fluids. Virus antigens were not detected for 25 days after frozen, primary HEL-12 cells were reinstated in culture. The cells expressed virus antigens but did not release virus particles between 25 and 80 days. Spontaneous virus release and maximal antigen expression occurred in cells grown for 80 to 120 days. Virus particles were not detected after 120 days although virus antigens persisted until the experiment was terminated. The HEL-12 virus was infectious for cell cultures of human, rhesus monkey, dog and rabbit cells. The proportion of SiSV-like and RD-114-like antigenic components of HEL-12 virus were altered by passage through heterologous cells suggesting heterogeneity of the HEL-12 virus population.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0022-1317
pubmed:author
pubmed:issnType
Print
pubmed:volume
35
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
487-95
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1977
pubmed:articleTitle
Cell generation and type C virus expression in the human embryonic cell strain HEL-12.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.