Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
23
pubmed:dateCreated
1983-3-11
pubmed:abstractText
An in vitro system in which bacteriophage T7 DNA is replicated and efficiently packaged into procapsids to form viable phage has been used to examine mutagenesis. The fidelity of replication was assayed both by measuring reversion of an amber mutation in an essential gene and by generation of temperature-sensitive mutants among the phage produced in vitro. Under standard reaction conditions, the fidelity of DNA replication is about equal to that normally found in vivo. However, when O6-methyldeoxyguanosine triphosphate is included in the reaction, O6-methylguanine is incorporated into newly synthesized DNA and the mutation frequencies increase 10- to 70-fold over the control. These experiments demonstrate in vitro mutagenesis with the T7 DNA replication-packaging system and provide more direct evidence for the premutagenic role of O6-methylguanine.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/6961422-276868, http://linkedlifedata.com/resource/pubmed/commentcorrection/6961422-327090, http://linkedlifedata.com/resource/pubmed/commentcorrection/6961422-330877, http://linkedlifedata.com/resource/pubmed/commentcorrection/6961422-373805, http://linkedlifedata.com/resource/pubmed/commentcorrection/6961422-416218, http://linkedlifedata.com/resource/pubmed/commentcorrection/6961422-4366872, http://linkedlifedata.com/resource/pubmed/commentcorrection/6961422-4554613, http://linkedlifedata.com/resource/pubmed/commentcorrection/6961422-4597463, http://linkedlifedata.com/resource/pubmed/commentcorrection/6961422-4706005, http://linkedlifedata.com/resource/pubmed/commentcorrection/6961422-4911848, http://linkedlifedata.com/resource/pubmed/commentcorrection/6961422-5791738, http://linkedlifedata.com/resource/pubmed/commentcorrection/6961422-6214209, http://linkedlifedata.com/resource/pubmed/commentcorrection/6961422-6245236, http://linkedlifedata.com/resource/pubmed/commentcorrection/6961422-6261110, http://linkedlifedata.com/resource/pubmed/commentcorrection/6961422-6749819, http://linkedlifedata.com/resource/pubmed/commentcorrection/6961422-691109, http://linkedlifedata.com/resource/pubmed/commentcorrection/6961422-6954535, http://linkedlifedata.com/resource/pubmed/commentcorrection/6961422-7000780, http://linkedlifedata.com/resource/pubmed/commentcorrection/6961422-7008792, http://linkedlifedata.com/resource/pubmed/commentcorrection/6961422-7022130, http://linkedlifedata.com/resource/pubmed/commentcorrection/6961422-7024247, http://linkedlifedata.com/resource/pubmed/commentcorrection/6961422-7040986, http://linkedlifedata.com/resource/pubmed/commentcorrection/6961422-7052056, http://linkedlifedata.com/resource/pubmed/commentcorrection/6961422-7143564, http://linkedlifedata.com/resource/pubmed/commentcorrection/6961422-768492
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:volume
79
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
7440-4
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1982
pubmed:articleTitle
Mutagenesis of bacteriophage T7 in vitro by incorporation of O6-methylguanine during DNA synthesis.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.