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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1983-1-7
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pubmed:abstractText |
Using the thymidine kinase gene-containing plasmid pTK-1 and the mouse fibroblast thymidine kinase-deficient LTK- cell line, we obtained results indicating that the plasmid molecules suspended in the external medium were introduced into the nuclei by pricking cells on the nuclei with a microneedle. Approx. 25% of the cells pricked in the presence of the plasmid pTK-1 showed TK gene expression, and 2% of the cells became stable TK+ transformants. This 'pricking' method is applicable for introducing DNA into cell nuclei and is more efficient than the conventional calcium phosphate transformation method.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
Nov
|
pubmed:issn |
0014-4827
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pubmed:author | |
pubmed:issnType |
Print
|
pubmed:volume |
142
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
79-84
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:6958471-Animals,
pubmed-meshheading:6958471-Cell Division,
pubmed-meshheading:6958471-Cells, Cultured,
pubmed-meshheading:6958471-DNA, Recombinant,
pubmed-meshheading:6958471-Genetic Engineering,
pubmed-meshheading:6958471-Mice,
pubmed-meshheading:6958471-Plasmids,
pubmed-meshheading:6958471-Thymidine Kinase,
pubmed-meshheading:6958471-Transformation, Genetic
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pubmed:year |
1982
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pubmed:articleTitle |
The 'pricking' method. A new efficient technique for mechanically introducing foreign DNA into the nuclei of culture cells.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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