Linked Life Data

6956714

Source:http://linkedlifedata.com/resource/pubmed/id/6956714

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
1982-12-2
pubmed:abstractText
BACTERIAL-NEUTROPHIL INTERACTIONS may be critical determinants of virulence in periodontal diseases. This study was undertaken to examine the ability of major bacterial species from the human oral cavity to inhibit (1) peripheral blood neutrophil chemotaxis, (2) chemotactic formylmethionyl peptide binding, and (3) phagocytosis. Included were cultured supernatants and sonic extracts obtained from strains of Capnocytophaga, Bacteroides gingivalis, Fusobacterium nucleatum, Bacteroides asaccharolyticus, Capnocytophaga species, Actinobacillus actinomycetemcomitans, Neisseria, Actinomyces viscosus, Bacterionema matruchotii, Streptococcus mitis, Streptococcus mutans, and Streptococcus sanguis. Chemotaxis was measured using Boyden chambers; phagocytosis was determined using Staphylococcus aureus as the indicator organism and radioactive chemotactic peptide binding was assessed by a rapid filtration assay. None of the test organisms were cytotoxic to neutrophils or inhibited neutrophil phagocytosis. Capnocytophaga species., Bacteroides species., A. actinomycetemcomitans, and F. nucleatum produced factors which specifically inhibited neutrophil chemotaxis. Activity was lost after dialysis. Extracts of Bacteroides species, A. actinomycetemcomitans and F. nucleatum, which were not chemotactic by themselves, inhibited binding of chemotactic peptide suggesting that in vitro chemotaxis inhibition was mediated by nonchemotactic components that compete for the chemotactic factor receptor on the neutrophil. The exception was Capnocytophaga which appeared to inhibit chemotaxis by inhibition of a post-binding event. Such chemotactic inhibitors from periodontopathic organisms that inhibit neutrophil function may be important determinants of virulence.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
D
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0022-3492
pubmed:author
pubmed:issnType
Print
pubmed:volume
53
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
502-8
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1982
pubmed:articleTitle
Inhibition of neutrophil chemotaxis by soluble bacterial products.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.