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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
8
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pubmed:dateCreated |
1982-8-26
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pubmed:abstractText |
If neoplastic transformation of diploid human cells results from carcinogen-induced mutations, cells deficient in excision repair of UV-induced DNA damage should be significantly more sensitive to transformation by UV light than normal cells. We tested this hypothesis by irradiating fibroblasts from a xeroderma pigmentosum patient (XP7BE, complementation group D) with low doses of Uv light (254 nm) and cells from a normal person with much higher doses and comparing the frequency of transformation to anchorage independence. Both sets of cells exhibited a dose-dependent increase in transformation which corresponded to a dose-dependent decrease in survival. At doses that caused equal cell killing, the frequency of anchorage-independent cells was approximately equal. Colonies of XP7BE and normal cells isolated from agar, propagated, and injected into X-irradiated athymic mice produced fibrosarcomas in 100% of the animals. Normal cells irradiated shortly before the onset of DNA synthesis exhibited a high frequency of anchorage-independent cells; cells irradiated in early G1 showed no increase over background. These results agree with those we observed for UV induction of 6-thioguanine-resistant mutants in these cells and support the hypothesis that anchorage independence results from mutations induced by DNA replication on a damaged template.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6953417-1061098,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6953417-262553,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6953417-291058,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6953417-4463168,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6953417-4811796,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6953417-503098,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6953417-7207499,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6953417-7214333,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6953417-7237421,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6953417-7249150,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6953417-7252419,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6953417-7371028,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6953417-865487,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6953417-934300
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
79
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2613-7
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:6953417-Cell Cycle,
pubmed-meshheading:6953417-Cell Line,
pubmed-meshheading:6953417-Cell Survival,
pubmed-meshheading:6953417-Cell Transformation, Neoplastic,
pubmed-meshheading:6953417-DNA Repair,
pubmed-meshheading:6953417-Dose-Response Relationship, Radiation,
pubmed-meshheading:6953417-Humans,
pubmed-meshheading:6953417-Infant, Newborn,
pubmed-meshheading:6953417-Male,
pubmed-meshheading:6953417-Skin,
pubmed-meshheading:6953417-Thioguanine,
pubmed-meshheading:6953417-Ultraviolet Rays,
pubmed-meshheading:6953417-Xeroderma Pigmentosum
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pubmed:year |
1982
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pubmed:articleTitle |
Frequency of UV-induced neoplastic transformation of diploid human fibroblasts is higher in xeroderma pigmentosum cells than in normal cells.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.
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