Linked Life Data

6950656

Source:http://linkedlifedata.com/resource/pubmed/id/6950656

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1982-5-21
pubmed:abstractText
Phytohaemagglutinin (PHA)-stimulated lymphocytes from healthy individuals and a malignant cell-line (K-562 cells) were cultured in a growth medium of 75 per cent RPMI 1640 (Gibco) supplemented with 25 per cent of uraemic serum or plasma obtained after systemic heparinization. Pooled human A Rh+ serum from 3 different donors served as controls. There was a depression of the DNA synthesis in both cell systems measured as uptake of methyl-3H-thymidine when the cells were cultured in uraemic milieu. Systemic heparinization markedly increased the depressive effect of uraemic plasma compared with controls. Heparin added to the growth media in concentrations much higher than those usually present in plasma after systemic heparinization had little effect on the cell proliferation. Systemic heparinization thus appears to induce production of toxic substances in plasma acting on proliferating cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0304-1328
pubmed:author
pubmed:issnType
Print
pubmed:volume
89
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
353-8
pubmed:dateRevised
2009-6-4
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
The effect of uraemic serum and plasma on proliferating cells in vitro.
pubmed:publicationType
Journal Article, Comparative Study, In Vitro, Research Support, Non-U.S. Gov't