|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
12
|
|
pubmed:dateCreated |
1982-5-21
|
|
pubmed:abstractText |
Monolayers of mouse peritoneal macrophages were shown to synthesize and secrete a protein that resembles apoprotein E (apoE), a normal constituent of plasma lipoproteins. Synthesis and secretion were studied by incubation of macrophages with L-[35S]methionine and analysis of the 35S-labeled proteins secreted into the culture medium. The 35S-labeled protein resembling apoE showed the following properties: (i) it floated in the ultracentrifuge at a density less than 1.215 g/ml, indicating that it was associated with lipid; (ii) by NaDodSO4/polyacrylamide gel electrophoresis, its Mr of 35,000 was identical to that of authentic apoE obtained from mouse plasma very low density lipoprotein; (iii) its isoelectric point of 5.4 was the same as that of authentic mouse apoE; (iv) it comigrated with authentic mouse apoE after two-dimensional isoelectric focusing/NaDodSO4/polyacrylamide gel electrophoresis; and (v) it was quantitatively precipitated by a monospecific antibody directed against rat apoE. Synthesis and secretion of the apoE-like protein was stimulated 3- to 8-fold when the macrophages were loaded with cholesterol by incubation with either acetylated low density lipoprotein (acetyl-LDL) or beta-migrating very low density lipoprotein from cholesterol-fed rabbits. When the cells were incubated with acetyl-LDL, the apoE-like protein composed approximately 2% of the total 35S-labeled protein synthesized by the cells and approximately 10% of the total 35S-labeled protein secreted into the medium. The current findings suggest a role for apoE in the plasma transport of cholesterol excreted from cholesterol-loaded macrophages.
|
|
pubmed:grant |
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6950395-13252080,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6950395-14907713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6950395-172422,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6950395-189822,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6950395-218198,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6950395-229107,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6950395-236308,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6950395-4354500,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6950395-6253476,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6950395-6255257,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6950395-6260135,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6950395-6261329,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6950395-6769124,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6950395-6933487,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6950395-7354059,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6950395-7354064,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6950395-7381331,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6950395-7410428,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6950395-7462813
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Dec
|
|
pubmed:issn |
0027-8424
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
78
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
7545-9
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:6950395-Animals,
pubmed-meshheading:6950395-Apolipoproteins,
pubmed-meshheading:6950395-Apolipoproteins E,
pubmed-meshheading:6950395-Ascitic Fluid,
pubmed-meshheading:6950395-Cholesterol,
pubmed-meshheading:6950395-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:6950395-Macrophages,
pubmed-meshheading:6950395-Male,
pubmed-meshheading:6950395-Mice,
pubmed-meshheading:6950395-Molecular Weight
|
|
pubmed:year |
1981
|
|
pubmed:articleTitle |
Mouse macrophages synthesize and secrete a protein resembling apolipoprotein E.
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|
