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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
11
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pubmed:dateCreated |
1982-2-12
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pubmed:abstractText |
The JB-6 mouse epidermal cell line has been used as a model system for studying the mechanism of late-stage promoter-dependent preneoplastic progression. The studies reported here are concerned with determining whether there is a requirement for mitogenic stimulation in promotion of anchorage independence and tumorigenicity in JB-6 cells. Such a requirement would predict that variants selected for 12-O-tetradecanoylphorbol 13-acetate (TPA) mitogen resistance would also be promotion resistant. Promotion-responsive cell lines have been selected for resistance to TPA-induced mitogenic stimulation at plateau density by cotreatment with colchicine and removal of mitogen-responsive colchicine-detached cells. The selected TPA-resistant population of cells showed a mitogenic response that was diminished by a factor of four but showed no diminution in the promotion-of-anchorage-independence response to TPA. Mitogen-resistant clonal lines derived from the selected population fell into three phenotypic classes when assayed in soft agar: (i) anchorage-independent transformants; (ii) variants resistant to promotion of anchorage independence by TPA; and (iii) variants sensitive to promotion by TPA. The existence of the latter class (i.e., the mitogen-resistant promotable variants) indicates a lack of obligatory causal relationship between TPa-induced mitogenesis and late-stage promotion and, thereby suggests that the two events can be dissociated.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6947266-11219849,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6947266-14150247,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6947266-195722,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6947266-302945,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6947266-314942,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6947266-4292180,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6947266-492322,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6947266-6119803,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6947266-626967,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6947266-6769125,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6947266-6774342,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6947266-6942676,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6947266-7371025,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6947266-7427940
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
78
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
6912-6
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:6947266-Animals,
pubmed-meshheading:6947266-Cell Line,
pubmed-meshheading:6947266-Cell Transformation, Neoplastic,
pubmed-meshheading:6947266-Clone Cells,
pubmed-meshheading:6947266-Colchicine,
pubmed-meshheading:6947266-Drug Resistance,
pubmed-meshheading:6947266-Genetic Variation,
pubmed-meshheading:6947266-Mice,
pubmed-meshheading:6947266-Mitogens,
pubmed-meshheading:6947266-Phenotype,
pubmed-meshheading:6947266-Phorbols,
pubmed-meshheading:6947266-Skin,
pubmed-meshheading:6947266-Tetradecanoylphorbol Acetate
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pubmed:year |
1981
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pubmed:articleTitle |
Dissociation of mitogenesis and late-stage promotion of tumor cell phenotype by phorbol esters: mitogen-resistant variants are sensitive to promotion.
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pubmed:publicationType |
Journal Article
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