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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
10
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pubmed:dateCreated |
1981-2-26
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pubmed:abstractText |
Total RNA, prepared from immature or mature mouse testes or from spermatogenic cells separated on the basis of sedimentation velocity, was translated in vitro. Mouse protamine-like histone could be identified as an in vitro translational product when [3H]arginine was used as the label. The mRNA for protamine-like histone was detected only after meiosis; the appearance of a peak of radioactivity comigrating with protamine-like histone occurred only when RNA from mature testes or late spermatid cell fractions was translated. Phosphoglycerate kinase-2 (ATP:3-phospho-D-glycerate I-phosphotransferase, EC 2.7.2.3) was identified as an in vitro translational product by affinity chromatography followed by two-dimensional gel electrophoresis or by specific immunoprecipitation when [35S]methionine was the label. The mRNA for phosphoglycerate kinase-2 was detected only in mature testes or late spermatid cell fractions. These translational assays for protamine-like histone and phosphoglycerate kinase-2 mRNAs suggest that these messages are transcribed after meiosis.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6934535-1095089,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6934535-1183756,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6934535-1204939,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6934535-1236854,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6934535-1254578,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6934535-14248592,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6934535-225240,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6934535-236308,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6934535-323095,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6934535-357186,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6934535-4109030,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6934535-422575,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6934535-4667689,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6934535-469836,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6934535-4850204,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6934535-4852488,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6934535-4950062,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6934535-5111021,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6934535-5345404,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6934535-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6934535-551176,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6934535-5615180,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6934535-620694,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6934535-648532,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6934535-658597,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6934535-6766938,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6934535-823012,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6934535-849423,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6934535-89113,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6934535-911755,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6934535-942653,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6934535-952894,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6934535-978173
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
77
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
6086-90
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:6934535-Animals,
pubmed-meshheading:6934535-Histones,
pubmed-meshheading:6934535-Male,
pubmed-meshheading:6934535-Meiosis,
pubmed-meshheading:6934535-Mice,
pubmed-meshheading:6934535-Phosphoglycerate Kinase,
pubmed-meshheading:6934535-Protamines,
pubmed-meshheading:6934535-RNA, Messenger,
pubmed-meshheading:6934535-Spermatogenesis,
pubmed-meshheading:6934535-Transcription, Genetic
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pubmed:year |
1980
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pubmed:articleTitle |
Quantitation of mRNAs during mouse spermatogenesis: protamine-like histone and phosphoglycerate kinase-2 mRNAs increase after meiosis.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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