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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1983-4-21
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pubmed:abstractText |
Rat parotid gland homogenates were fractionated into mitochondrial, heavy microsomal and light microsomal fractions by differential centrifugation. ATP-dependent 45Ca2+ uptake by the subcellular fractions paralleled the distribution of NADPH-cytochrome c reductase, an enzyme associated with the endoplasmic reticulum. The highest rate of Ca2+ uptake was found in the heavy microsomal fraction. Ca2+ uptake by this fraction was dependent on the presence of ATP and was sustained at a linear rate by 5 mM-oxalate. Inhibitors of mitochondrial Ca2+ transport had no effect on the rate of Ca2+ uptake. Na+ and K+ stimulated Ca2+ uptake. At optimal concentrations. Na+ stimulated Ca2+ uptake by 120% and K+ stimulated Ca2+ uptake by 260%. Decreasing the pH from 7.4 to 6.8 had little effect on Ca2+ uptake. The Km for Ca2+ uptake was 3.7 microM free Ca2+ and 0.19 mM-ATP. Vanadate inhibited Ca2+ uptake; 60 microM-vanadate inhibited the rate of Ca2+ accumulation by 50%. It is concluded that the ATP-dependent Ca2+ transport system is located on the endoplasmic reticulum and may play a role in maintaining intracellular levels of free Ca2+ within a narrow range of concentration.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6925974-11219,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6925974-1211449,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6925974-13271416,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6925974-140870,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6925974-174440,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6925974-189753,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6925974-193840,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6925974-209290,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6925974-215892,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6925974-218805,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6925974-23291,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6925974-4245535,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6925974-4249366,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6925974-4314708,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6925974-4468821,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6925974-5246541,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6925974-6106020,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6925974-6158259,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6925974-6258180,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6925974-6258447,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6925974-6449671,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6925974-6451626,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6925974-7067823,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6925974-806589
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
208
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
789-94
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:6925974-Adenosine Triphosphate,
pubmed-meshheading:6925974-Animals,
pubmed-meshheading:6925974-Biological Transport, Active,
pubmed-meshheading:6925974-Calcium,
pubmed-meshheading:6925974-Kinetics,
pubmed-meshheading:6925974-Male,
pubmed-meshheading:6925974-Microsomes,
pubmed-meshheading:6925974-Parotid Gland,
pubmed-meshheading:6925974-Potassium,
pubmed-meshheading:6925974-Rats,
pubmed-meshheading:6925974-Rats, Inbred Strains,
pubmed-meshheading:6925974-Sodium,
pubmed-meshheading:6925974-Subcellular Fractions,
pubmed-meshheading:6925974-Vanadates,
pubmed-meshheading:6925974-Vanadium
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pubmed:year |
1982
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pubmed:articleTitle |
Parotid microsomal Ca2+ transport. Subcellular localization and characterization.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't
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