Linked Life Data

6919541

Source:http://linkedlifedata.com/resource/pubmed/id/6919541

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
13
pubmed:dateCreated
1982-8-26
pubmed:abstractText
Two human tRNAiMet genes were previously cloned from a recombinant library of fetal liver DNA (Santos, T., and Zasloff, M. (1981) Cell 23, 699-709). One gene differed from the common vertebrate sequence by a G to T transversion at position 56, occupied exclusively by a purine in all prokaryotic and eukaryotic tRNAs. In this study, we show that although both tRNAiMet genes are transcribed in an in vitro system from KB cells, normal post-transcriptional processing of the mutant gene transcript is interrupted. While the primary transcript of the normal gene undergoes stepwise excision of its 5' and 3' terminal sequences, 5' preceding 3', only the 5' leader of the primary transcript of the mutant gene is excised, resulting in the accumulation of an intermediate containing an unprocessed 3' trailer. The results suggest that certain eukaryotic tRNA mutations may not appear in mature tRNA species due to the effects of the mutation on precursor processing.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
10
pubmed:volume
257
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
7857-63
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1982
pubmed:articleTitle
Transcription and precursor processing of normal and mutant human tRNAiMet genes in a homologous cell-free system.
pubmed:publicationType
Journal Article, Comparative Study