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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1982-1-9
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pubmed:abstractText |
1. Dimethylnitrosamine (37.5 mg/kg body wt.) was administered to mice by a single intraperitoneal injection, and the early effects on protein synthesis and related functions were studied in a liver S-30 system. 2. The incorporation of [14C]leucine into protein decreased rapidly after dimethylnitrosamine administration. The effect was associated with a decreased ability of the system to utilize methionyl-tRNAfMet and formyl-methionyl-tRNAfMet for 80 S ribosomal initiation-complex formation (primary initiation), and a loss of poly(A)-containing RNA from the postmicrosomal fraction. All the three effects developed simultaneously, and were clearly demonstrable within 15 min. 3. Initiation-complex formation in the polyribosomal fraction (re-initiation) was decreased to the same extent as the primary initiation, indicating that the initiation defect was not a result of the decrease in free mRNA. 4. The inhibition of initiation was only manifest at the joining of the 40 S pre-initiation complex to 60 S ribosomal subunits. It was not a result of methionyl-tRNAfMet deacylation. The functions between the formation of the methionyl-tRNAfMet-containing 80 S ribosomal complex and the first translocation on the ribosome were not involved, since the incorporation of formylmethionine into N-terminal polypeptides decreased to the same extent as the 80 S initiation-complex formation. 5. Inhibitors of protein synthesis (cycloheximide and pactamycin) decreased poly(A)-containing RNA in the postmicrosomal fraction in a similar way to dimethylnitrosamine.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6914198-1053014,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6914198-1132481,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6914198-1148247,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6914198-1201618,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6914198-13607415,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6914198-13804867,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6914198-14240664,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6914198-14403971,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6914198-175964,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6914198-326002,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6914198-372180,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6914198-4111381,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6914198-4605134,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6914198-4703560,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6914198-4721235,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6914198-4732074,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6914198-476665,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6914198-4778368,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6914198-4915406,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6914198-5011971,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6914198-5072706,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6914198-5120304,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6914198-5144259,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6914198-5584005,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6914198-559547,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6914198-5772121,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6914198-598401,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6914198-6272731,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6914198-647838,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6914198-837928,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6914198-942051,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6914198-972853
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
194
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
469-74
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:6914198-Animals,
pubmed-meshheading:6914198-Dimethylnitrosamine,
pubmed-meshheading:6914198-Liver,
pubmed-meshheading:6914198-Male,
pubmed-meshheading:6914198-Mice,
pubmed-meshheading:6914198-Peptide Biosynthesis,
pubmed-meshheading:6914198-Peptide Chain Initiation, Translational,
pubmed-meshheading:6914198-Poly A,
pubmed-meshheading:6914198-Protein Biosynthesis,
pubmed-meshheading:6914198-RNA, Transfer, Amino Acyl,
pubmed-meshheading:6914198-Ribosomes,
pubmed-meshheading:6914198-Subcellular Fractions,
pubmed-meshheading:6914198-Time Factors
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pubmed:year |
1981
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pubmed:articleTitle |
Early effects of dimethylnitrosamine on the initiation of protein synthesis in mouse liver.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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