Linked Life Data

6912099

Source:http://linkedlifedata.com/resource/pubmed/id/6912099

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1981-11-22
pubmed:abstractText
Haemopoietically active long-term bone marrow cultures from several species have been investigated ultrastructurally. Human, tree shrew and mouse cultures generally support granulopoiesis, although recently it has been possible to convert a granulopoietic mouse culture to extensive erythropoiesis. The haemopoietic products of the cultures include granulocytes (neutrophil and basophil), mast cells, monocytes, megakaryocytes and all stages of the erythrocytic series. Plasmacytes and occasional lymphocytes have been observed in small numbers in human cultures (possible indicating retention rather than formation). The stromal elements of the adherent layer of these cultures include endothelial cells, reticulum cells, fat cells and fibroblasts. The adherent layers are responsible for the inductive microenvironment within the cultures, and show features specific for the line of differentiation. In the granulocytic cultures there is close association between developing fat cells (reticulum cells) and granulocyte precursors. Endothelial cell monolayers cover large regions of these cultures, and the areas beneath this monolayer are rich in early granulocytes. Mature granulocytes and monocytes migrate through the endothelial layer, demonstrating in vitro "transmural passage". Cultures stimulated for erythropoiesis show a considerable reduction in fat cells, in endothelial cell cover and in the numbers of classical monocytes. Erythropoiesis appears to be promoted by a close association of the entire erythrocytic series with monocytic cells, forming "erythroblastic islets" in vitro. A possible pathway of intracellular communication between differentiating haemopoietic cells and the stromal cells in their microenvironment is suggested.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:issn
0300-5208
pubmed:author
pubmed:issnType
Print
pubmed:volume
84
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
38-67
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
Haemopoietic microenvironments in vitro: ultrastructural aspects.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't