Linked Life Data

690132

Source:http://linkedlifedata.com/resource/pubmed/id/690132

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
19
pubmed:dateCreated
1978-11-29
pubmed:abstractText
The affinity (Ka) of human coagulation Factor Xa for thrombin-treated (to stimulate the release reaction) platelets has been determined to be 3 to 4 x 10(10) M-1 by equilibrium binding studies using 125I-labeled Xa. The binding of Factor Xa to platelets results in an increase of 300,000-fold in the apparent enzymatic activity of Xa in the conversion of prothrombin to thrombin. The activity of platelet surface Xa is approximately 15-fold greater than that observed with optimum concentrations of bovine Factor V and phospholipids in place of platelets. Ca2+ is required for the Xa-platelet interaction; the optimum concentration is 2.5 mM. Related coagulation factors, including Factor X, Factor IXa, diisopropylphosphoryl Factor Xa, and prothrombin do not complete with Factor Xa for the Xa binding sites. The rate of thrombim formation at saturating amounts of Xa is directly proportional to the number of platelets from 1 x 10(7) to 5 x 10(8) platelets/ml. Factor Xa bound to platelets is not inactivated by antithrombin III. An antibody that inhibits both human and bovine coagulation Factor V activity blocks both Xa binding to released platelets and the rapid thrombin formation associated with this binding, suggesting that Factor V from platelets is involved in the Xa-platelet interaction.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
10
pubmed:volume
253
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
6908-16
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1978
pubmed:articleTitle
Properties of the factor Xa binding site on human platelets.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.