Linked Life Data

6895725

Source:http://linkedlifedata.com/resource/pubmed/id/6895725

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1982-4-22
pubmed:abstractText
PRL production in primary cultures of rat pituitary cells was examined for evidence of autoregulation. The kinetics of PRL accumulation in the culture medium were linear (r = 0.993) over a 20-fold range of PRL concentrations (85-1800 ng/ml). The addition of 0.01-1000 ng/ml biologically active ovine PRL for 2 h had no effect on the rate of secretion or the intracellular levels of PRL. The functional integrity of the cells was verified by the demonstration that estradiol (10(-8) M) caused a 3-fold increase in both PRL synthesis and secretion. Ergocryptine (10(-8) M) inhibited PRL secretion by 57% (P less than 0.001), and this response was modulated by pretreatment with estradiol. Increasing concentrations of endogenously secreted PRL did not detectably alter the rate of incorporation of [35S]methionine into cellular PRL in control or ergocryptine-treated cells for up to 8 h. These results indicate that autoregulation of PRL does not operate directly at the level of the pituitary.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0013-7227
pubmed:author
pubmed:issnType
Print
pubmed:volume
110
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
722-6
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1982
pubmed:articleTitle
Evidence that autoregulation of prolactin production does not occur at the pituitary level.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't