Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1981-12-21
pubmed:abstractText
Late in terminal differentiation, human epidermal keratinocytes form an insoluble protein envelope on the cytoplasmic side of the plasma membrane. Involucrin, a soluble protein precursor of the envelope, is synthesized at an earlier stage of differentiation, both in the natural epithelium and in cultured keratinocytes. Because keratinocytes are known to enlarge during differentiation, we looked for a correlation between involucrin synthesis and cell size, using antiserum raised against the purified protein. We found that virtually no cultured epidermal keratinocytes with a diameter less than or equal to 14 micrometer contained involucrin, but most cells greater than 17 micrometer did. Using density gradient centrifugation, we were able to isolate a population of small cells containing almost no involucrin, as judged by immunodiffusion, PAGE, and immunoprecipitation. Large cells possessed translatable mRNA for involucrin, whereas small cells did not. We conclude that when cultured keratinocytes reach a certain size (approximately 14 micrometer in diameter) the specific mRNA for involucrin begins to accumulate and synthesis of the protein begins.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/6895226-1009573, http://linkedlifedata.com/resource/pubmed/commentcorrection/6895226-1052771, http://linkedlifedata.com/resource/pubmed/commentcorrection/6895226-1100551, http://linkedlifedata.com/resource/pubmed/commentcorrection/6895226-1175627, http://linkedlifedata.com/resource/pubmed/commentcorrection/6895226-172344, http://linkedlifedata.com/resource/pubmed/commentcorrection/6895226-187693, http://linkedlifedata.com/resource/pubmed/commentcorrection/6895226-24643, http://linkedlifedata.com/resource/pubmed/commentcorrection/6895226-299924, http://linkedlifedata.com/resource/pubmed/commentcorrection/6895226-383305, http://linkedlifedata.com/resource/pubmed/commentcorrection/6895226-42494, http://linkedlifedata.com/resource/pubmed/commentcorrection/6895226-4516600, http://linkedlifedata.com/resource/pubmed/commentcorrection/6895226-4636326, http://linkedlifedata.com/resource/pubmed/commentcorrection/6895226-4850204, http://linkedlifedata.com/resource/pubmed/commentcorrection/6895226-4850663, http://linkedlifedata.com/resource/pubmed/commentcorrection/6895226-5100767, http://linkedlifedata.com/resource/pubmed/commentcorrection/6895226-5396942, http://linkedlifedata.com/resource/pubmed/commentcorrection/6895226-5432063, http://linkedlifedata.com/resource/pubmed/commentcorrection/6895226-6155214, http://linkedlifedata.com/resource/pubmed/commentcorrection/6895226-6160118, http://linkedlifedata.com/resource/pubmed/commentcorrection/6895226-6895225, http://linkedlifedata.com/resource/pubmed/commentcorrection/6895226-6999941, http://linkedlifedata.com/resource/pubmed/commentcorrection/6895226-823012, http://linkedlifedata.com/resource/pubmed/commentcorrection/6895226-83196, http://linkedlifedata.com/resource/pubmed/commentcorrection/6895226-837449, http://linkedlifedata.com/resource/pubmed/commentcorrection/6895226-890737, http://linkedlifedata.com/resource/pubmed/commentcorrection/6895226-89113
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0021-9525
pubmed:author
pubmed:issnType
Print
pubmed:volume
90
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
738-42
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
Involucrin synthesis is correlated with cell size in human epidermal cultures.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.