Linked Life Data

6885993

Source:http://linkedlifedata.com/resource/pubmed/id/6885993

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1983-10-8
pubmed:abstractText
We compared two methods for quantitating antigen-specific antibody by a reverse enzyme-linked immunosorbent assay. In the serial-dilution method, the result was determined to be the highest dilution of a serum yielding an absorbance above an established threshold. In the single-dilution method, the result was determined by comparing the absorbance yielded by the test serum at a standard dilution to that yielded by positive and negative reference sera at the same dilution. The results in the single-dilution method reflected the antigen-specific immunoglobulin M antibody activity as a proportion of total immunoglobulin M antibody in a serum, i.e., the immune load, whereas the results in the serial-dilution method reflected the absolute concentration of antigen-specific immunoglobulin M antibody activity. Compared with results in the serial-dilution method, results in the single-dilution method had considerably greater reproducibility on a day-to-day basis and under various test conditions. The single-dilution method was more useful in discriminating between sera from patients in an early stage of clinical infection due to Toxoplasma gondii and sera from patients in a later stage of infection.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/6885993-231096, http://linkedlifedata.com/resource/pubmed/commentcorrection/6885993-395275, http://linkedlifedata.com/resource/pubmed/commentcorrection/6885993-5135623, http://linkedlifedata.com/resource/pubmed/commentcorrection/6885993-6154109, http://linkedlifedata.com/resource/pubmed/commentcorrection/6885993-6172520, http://linkedlifedata.com/resource/pubmed/commentcorrection/6885993-6270191, http://linkedlifedata.com/resource/pubmed/commentcorrection/6885993-7000834, http://linkedlifedata.com/resource/pubmed/commentcorrection/6885993-7007520, http://linkedlifedata.com/resource/pubmed/commentcorrection/6885993-7021589, http://linkedlifedata.com/resource/pubmed/commentcorrection/6885993-7077100, http://linkedlifedata.com/resource/pubmed/commentcorrection/6885993-7452400, http://linkedlifedata.com/resource/pubmed/commentcorrection/6885993-80616
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0095-1137
pubmed:author
pubmed:issnType
Print
pubmed:volume
18
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
63-70
pubmed:dateRevised
2010-9-10
pubmed:meshHeading
pubmed:year
1983
pubmed:articleTitle
Comparison of methods for quantitating antigen-specific immunoglobulin M antibody with a reverse enzyme-linked immunosorbent assay.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't