Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1983-10-8
pubmed:abstractText
Plasma gonadotropin (GtH) and 17 alpha, 20 beta-dihydroxy-4-pregnen-3-one (17 alpha, 20 beta-diOH-prog) levels were assessed by radioimmunoassay during the sexual maturation of female amago salmon (Oncorhynchus rhodurus). Both GtH and 17 alpha, 20 beta-diOHprog levels were low in vitellogenic females (June to September) and in those with full-grown immature oocytes and were elevated in mature and ovulated females to 40 ng/ml for GtH and 50-70 ng/ml for 17 alpha, 20 beta-diOHprog. In vitro production of 17 alpha, 20 beta-diOHprog by ovarian follicles and its stimulation by partially purified chinook salmon gonadotropin (SG-G100) was examined monthly using 18 hr incubations. In June and July, levels were too low to detect (less than 30 pg/ml) in media from all treatment groups. In August and September, SG-G100 at a concentration of 1 microgram/ml stimulated low levels of production (0.2-0.3 ng/ml). Full-grown, immature follicles in October showed a dose-response production of 17 alpha, 20 beta-diOHprog, averaging over 10 ng/ml when incubated with 1 microgram/ml SG-G100. Postovulatory follicles (1-2 days after ovulation) produced large amounts of 17 alpha, 20 beta-diOHprog, averaging over 100 ng/ml with 1 microgram/ml SG-G100. These results are discussed in relation to previous work on amago salmon and other species and together indicate that 17 alpha, 20 beta-diOHprog is the major steroid responsible for oocyte maturation in amago salmon, produced as the follicular mediator of gonadotropin.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0016-6480
pubmed:author
pubmed:issnType
Print
pubmed:volume
51
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
96-105
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1983
pubmed:articleTitle
Plasma 17 alpha, 20 beta-dihydroxy-4-pregnen-3-one levels during sexual maturation of amago salmon (Oncorhynchus rhodurus): correlation with plasma gonadotropin and in vitro production by ovarian follicles.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't