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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
|
pubmed:dateCreated |
1983-10-8
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pubmed:abstractText |
Human late prophase to late metaphase chromosomes were prepared after amethopterin cell synchronization. R-banding was produced by heat denaturation followed by Giemsa staining (RHG). Haploid sets of prophase chromosomes contain approximately 850 bands. Sequences of chromosomes of different degrees of condensation are presented; their analysis provides helpful information to identify the elongated chromosomes and to follow band subdivision. The heat denaturation technique is free from most of the disadvantages encountered with R-banding by 5-bromodeoxyuridine incorporation. Giemsa stained R-bands produced by heat denaturation on prophase and prometaphase chromosomes are useful to analyse the numerous chromosome anomalies involving R-bands. In conjunction with G-banding, it is also important to compare adequately the positive and negative regions of each chromosome to define the anomalies with precision.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
Jun
|
pubmed:issn |
0008-4093
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pubmed:author | |
pubmed:issnType |
Print
|
pubmed:volume |
25
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
261-9
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:6883179-Azure Stains,
pubmed-meshheading:6883179-Chromosome Banding,
pubmed-meshheading:6883179-Chromosomes, Human,
pubmed-meshheading:6883179-Hot Temperature,
pubmed-meshheading:6883179-Humans,
pubmed-meshheading:6883179-Metaphase,
pubmed-meshheading:6883179-Methotrexate,
pubmed-meshheading:6883179-Nucleic Acid Denaturation,
pubmed-meshheading:6883179-Prophase
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pubmed:year |
1983
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pubmed:articleTitle |
R-banding of human chromosomes by heat denaturation and Giemsa staining after amethopterin-synchronization.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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