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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
8
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pubmed:dateCreated |
1983-10-8
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pubmed:abstractText |
A monoclonal antibody (D-547) against human breast cancer oestrogen receptor was used to demonstrate that the oestrogen receptor in ovarian epithelial carcinomata binds [3H]monohydroxytamoxifen. This binding was observed in sedimentation velocity analyses of both cytosol and nuclear compartments. Binding of [3H]monohydroxytamoxifen to the antibody-recognized moiety could be inhibited by diethylstilboestrol or oestradiol, but not by androgen, progestin or corticoid, indicating oestrogen specificity for the binding site. The sedimentation coefficient of the antibody-recognized [3H]monohydroxytamoxifen binding moiety was similar to that when 16 alpha-[125I]iodo-oestradiol was used as the ligand.
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pubmed:grant |
|
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pubmed:language |
eng
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pubmed:journal |
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|
pubmed:citationSubset |
AIM
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pubmed:chemical |
|
|
pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0306-5456
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
90
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
751-8
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:6882707-Antibodies, Monoclonal,
pubmed-meshheading:6882707-Centrifugation, Density Gradient,
pubmed-meshheading:6882707-Estradiol,
pubmed-meshheading:6882707-Estrogen Antagonists,
pubmed-meshheading:6882707-Female,
pubmed-meshheading:6882707-Humans,
pubmed-meshheading:6882707-Ovarian Neoplasms,
pubmed-meshheading:6882707-Receptors, Estrogen,
pubmed-meshheading:6882707-Tamoxifen
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pubmed:year |
1983
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pubmed:articleTitle |
Binding of [3H] monohydroxytamoxifen in ovarian carcinoma.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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