Linked Life Data

6882406

Source:http://linkedlifedata.com/resource/pubmed/id/6882406

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2-3
pubmed:dateCreated
1983-9-9
pubmed:abstractText
The nearest neighbor relationships of major structural proteins within type D retroviruses (SMRV, MPMV, PMFV) were investigated by crosslinking with the cleavable bifunctional reagent dimethyl dithiobispropionimidate (DTBPI) or by use of 2-iminothiolane (methyl mercaptobutyrimidate, MBI) and subsequent oxidation by hydrogen peroxide. The crosslinked complexes of proteins were analyzed by two-dimensional diagonal polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. In intact virions both crosslinking reagents induced the formation of covalently linked complexes of the internal structural proteins as well as complexes containing the envelope glycoproteins. The following complexes were found for each virus: SMRV: (pp20)2; (p35)2,4,6; (pp20-gp68); MPMV: (p10)2,4,6; (p25)2,4; (p10-p25); (gp20-gp68); (p45-gp68); PMFV: (p10)2; (p25)2; (p10-p25); (p45-gp68). In control experiments without crosslinking reagents no covalently linked complexes were detected.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0232-766X
pubmed:author
pubmed:issnType
Print
pubmed:volume
42
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
177-87
pubmed:dateRevised
2000-12-18
pubmed:meshHeading
pubmed:year
1983
pubmed:articleTitle
Chemical crosslinking of major structural proteins within type D retroviruses.
pubmed:publicationType
Journal Article