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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1983-9-20
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pubmed:abstractText |
A factor which is responsible for the growth inhibitory properties of certain mouse sera and related to NK-activity, has been studied. The factor was isolated from hybrid B6D2F1 (C57Bl/6 x DBA/2) serum, which is histo-compatible with the mouse tumour (B16 melanoma) used and has high NK-activity. Growth inhibitory activity was measured in an in vitro assay. It was independent of complement activation. The responsive factor was isolated and characterized by ion exchange chromatography. Concanavalin A affinity chromatography, gel filtration and iso-electric focusing. It appears to be a protein and had been labelled growth-inhibitory factor (GIF). It has a molecular weight of 230 000-260 000 daltons and an iso-electric point in the pH range 4.6-5.0. It was not retained on Concanavalin A columns.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0108-0202
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
91
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
129-35
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pubmed:dateRevised |
2009-6-4
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pubmed:meshHeading |
pubmed-meshheading:6880747-Animals,
pubmed-meshheading:6880747-Cell Line,
pubmed-meshheading:6880747-Cobra Venoms,
pubmed-meshheading:6880747-Complement Activation,
pubmed-meshheading:6880747-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:6880747-Growth Inhibitors,
pubmed-meshheading:6880747-HeLa Cells,
pubmed-meshheading:6880747-Melanoma,
pubmed-meshheading:6880747-Membrane Proteins,
pubmed-meshheading:6880747-Mice,
pubmed-meshheading:6880747-Mice, Inbred C57BL,
pubmed-meshheading:6880747-Mice, Inbred DBA,
pubmed-meshheading:6880747-Molecular Weight
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pubmed:year |
1983
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pubmed:articleTitle |
Purification and characterization of a mouse serum protein with growth inhibitory properties in vitro against tumor cell lines.
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pubmed:publicationType |
Journal Article
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