Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1983-9-9
pubmed:abstractText
Replacement of enriched CMRL 1066 medium by cell-free ascites from tumour patients in the human tumour clonogenic assay described by Hamburger and Salmon (1977) increased plating efficiency for ovarian cancer cells by a median of 8-fold (range 0.4-1012 fold). In 40 experiments, two cases had a lower plating efficiency when cultured in cell-free ascites, 10 grew neither in standard medium nor in cell-free ascites and in two cases, growth was only observed in cell-free ascites. With standard medium, we observed 53% growth (greater than 5 colonies/dish) and 41% evaluable for chemosensitivity testing (greater than 30 colonies/dish). With cell-free ascites as culture medium, these figures were 71% and 63%, respectively. While under standard conditions the highest plating efficiency obtained was 0.25%, in 21% of the experiments done with cell-free ascites a plating efficiency higher than 1% was reached. We conclude that cell-free ascites is able to stimulate proliferation of ovarian cancer cells in agar and that the use of it extends the applicability of the clonogenic assay.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/6871079-210939, http://linkedlifedata.com/resource/pubmed/commentcorrection/6871079-302265, http://linkedlifedata.com/resource/pubmed/commentcorrection/6871079-343804, http://linkedlifedata.com/resource/pubmed/commentcorrection/6871079-427794, http://linkedlifedata.com/resource/pubmed/commentcorrection/6871079-6274537, http://linkedlifedata.com/resource/pubmed/commentcorrection/6871079-6946877, http://linkedlifedata.com/resource/pubmed/commentcorrection/6871079-6974274, http://linkedlifedata.com/resource/pubmed/commentcorrection/6871079-7234870, http://linkedlifedata.com/resource/pubmed/commentcorrection/6871079-7295510, http://linkedlifedata.com/resource/pubmed/commentcorrection/6871079-7318146, http://linkedlifedata.com/resource/pubmed/commentcorrection/6871079-7348571, http://linkedlifedata.com/resource/pubmed/commentcorrection/6871079-7459227, http://linkedlifedata.com/resource/pubmed/commentcorrection/6871079-77475
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0007-0920
pubmed:author
pubmed:issnType
Print
pubmed:volume
48
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
55-9
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1983
pubmed:articleTitle
Human tumour clonogenicity in agar is improved by cell-free ascites.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't