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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1983-5-5
|
| pubmed:abstractText |
Fluorogenic oligosaccharide derivatives, lactityl-aminopyridine and sialyllactityl-aminopyridines, were synthesized by reductive amination of lactose and sialyllactose with 2-aminopyridine in the presence of sodium cyanoborohydride. Lactityl-aminopyridine showed bluish-white fluorescence at 390 nm by excitation with ultraviolet light at 320 nm, and the intensity of the fluorescence was proportional to the concentration of lactityl-aminopyridine within a range from 20 to 9800 pmole/ml. Two isomers of sialyllactityl-aminopyridine were enzymatically hydrolyzed to sialic acid and lactityl-aminopyridine by the action of both microbial and mammalian sialidases, and their suitability as substrates for sialidase was investigated. The result showed that sialidase activity was determined with about 55-fold higher sensitivity than that of ordinarily used colorimetric methods.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Feb
|
| pubmed:issn |
0006-2944
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
29
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
23-30
|
| pubmed:dateRevised |
2009-11-11
|
| pubmed:meshHeading | |
| pubmed:year |
1983
|
| pubmed:articleTitle |
A sensitive assay of sialidase activity with fluorogenic oligosaccharide derivatives.
|
| pubmed:publicationType |
Journal Article
|