Linked Life Data

6848508

Source:http://linkedlifedata.com/resource/pubmed/id/6848508

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1983-2-14
pubmed:abstractText
Villin, a Ca2+-modulated F-actin-binding protein (95,000 daltons) present in microvillus core filament bundles, has been shown to contain multiple Ca2+-binding sites. 45Ca Hummel-Dreyer chromatography reveals the presence of two rapidly exchanging Ca2+-binding sites with an apparent dissociation constant, Kd, equal to 4.6 X 10(-6) M. Use of the two proteolytically separable domains of the molecule revealed that one site is located on the 90,000-dalton core (apparent Kd = 3.5 X 10(-6) M) while the second site is provided by the 8,800-dalton headpiece fragment (apparent Kd = 7.4 X 10(-6) M). In addition villin displays a further very slowly exchanging or nonexchangeable high affinity Ca2+-binding site, which is situated in the core domain. Secondary structural predictions and a comparison of the amino acid sequence of headpiece with other known Ca2+-binding proteins indicates one region suggestive of a Ca2+-binding site, although headpiece seems not to exhibit a classical "EF-hand" Ca2+-binding structure.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
10
pubmed:volume
258
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
365-9
pubmed:dateRevised
2003-11-14
pubmed:meshHeading
pubmed:year
1983
pubmed:articleTitle
Demonstration of three distinct calcium-binding sites in villin, a modulator of actin assembly.
pubmed:publicationType
Journal Article