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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1983-6-10
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pubmed:abstractText |
Electron transfer flavoprotein (ETF) from pig liver mitochondria has been purified to homogeneity by a three-step procedure with approx. 10-fold higher yields than previously reported. The purified ETF exhibits an absorption coefficient for the bound FAD of 13.5 mM-1.cm-1 at 436 nm and an isoelectric point of 6.75. Gel filtration, sodium dodecyl sulphate gel electrophoresis and flavin analysis indicate that pig liver ETF is a dimer, composed of non-identical subunits (Mr 38 000 and 32 000) with only one FAD/dimer. Anaerobic reduction by dithionite produces anionic flavin semiquinone as a stable intermediate and establishes the flavin to be the only redox-active chromophore in ETF.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6847633-1168197,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6847633-13295225,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6847633-13895406,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6847633-14240539,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6847633-207689,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6847633-374972,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6847633-4976788,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6847633-5691970,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6847633-570409,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6847633-5806584,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6847633-6049437,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6847633-6180732,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6847633-6930657,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6847633-7334008
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
209
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
541-5
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:6847633-Amino Acids,
pubmed-meshheading:6847633-Animals,
pubmed-meshheading:6847633-Chemical Phenomena,
pubmed-meshheading:6847633-Chemistry,
pubmed-meshheading:6847633-Dithionite,
pubmed-meshheading:6847633-Electron-Transferring Flavoproteins,
pubmed-meshheading:6847633-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:6847633-Flavoproteins,
pubmed-meshheading:6847633-Isoelectric Focusing,
pubmed-meshheading:6847633-Macromolecular Substances,
pubmed-meshheading:6847633-Mitochondria, Liver,
pubmed-meshheading:6847633-Spectrophotometry,
pubmed-meshheading:6847633-Swine
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pubmed:year |
1983
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pubmed:articleTitle |
Electron transfer flavoprotein from pig liver mitochondria. A simple purification and re-evaluation of some of the molecular properties.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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