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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
|
pubmed:dateCreated |
1983-6-10
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pubmed:abstractText |
Mouse lymphoblasts generated in vivo by a topical application of the contact sensitizer oxazolone or by the contents of the gut lumen were separated by discontinuous density centrifugation on Percoll gradients. A 3-step gradient was used to divide the cells into two subpopulations. For cells from oxazolone stimulated lymph nodes, the low density band contained 20-30% of the initial cell number applied to the gradient; 25-40% of this population were in S phase and nearly all the large and pyroninophilic cells were confined to this layer. The high density step cells (70-80% of initial cell number) were predominantly small lymphocytes with less than 0.5% in S phase. Similar results were obtained using cells from picryl chloride stimulated lymph nodes or from mesenteric lymph nodes.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0022-1759
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
29
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pubmed:volume |
59
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
159-65
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:6841977-Animals,
pubmed-meshheading:6841977-Cell Separation,
pubmed-meshheading:6841977-Centrifugation, Density Gradient,
pubmed-meshheading:6841977-Lymph Nodes,
pubmed-meshheading:6841977-Lymphocyte Activation,
pubmed-meshheading:6841977-Lymphocytes,
pubmed-meshheading:6841977-Mesentery,
pubmed-meshheading:6841977-Mice,
pubmed-meshheading:6841977-Mice, Inbred BALB C,
pubmed-meshheading:6841977-Mice, Inbred CBA,
pubmed-meshheading:6841977-Oxazolone
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pubmed:year |
1983
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pubmed:articleTitle |
Separation of mouse lymphoblasts by discontinuous density centrifugation on Percoll gradients.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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