Linked Life Data

6841352

Source:http://linkedlifedata.com/resource/pubmed/id/6841352

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1983-6-17
pubmed:abstractText
A rapid and simplified method for the preparation of lysosomal membranes from rat livers was developed. Enzymic characterization showed that the lysosomal membrane preparation isolated by this procedure was almost free from mitochondria, peroxisomes, and endoplasmic reticulum. Acid phosphatase was used as a marker enzyme for lysosomal membrane, because about 40% of the total acid phosphatase activity in the lysosomes was associated with the membranes. With this method, the yield of the purified membrane was 115 micrograms/g wet weight of liver and the relative specific activity of acid phosphatase in the purified membrane was 105-110. On SDS-polyacrylamide gel electrophoretic analysis, the purified membrane revealed glycoprotein bands in the region of M.W. between 60,000 and 110,000, which were characteristic of the tritosomal membrane. Our lysosomal membrane preparation was compared with lysosomal membranes prepared from normal rat liver lysosomes isolated by the method of Wattiaux et al. [(1978) J. Cell Biol. 78, 349-368]. Although the specific activity of acid phosphatase in our membrane preparation was higher than that in the membrane prepared by the existing method, the electrophoretic profiles of both membrane preparations were quite similar.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0021-924X
pubmed:author
pubmed:issnType
Print
pubmed:volume
93
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
547-56
pubmed:dateRevised
2007-12-19
pubmed:meshHeading
pubmed:year
1983
pubmed:articleTitle
A rapid and simplified method for the preparation of lysosomal membranes from rat liver.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't