|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
2
|
|
pubmed:dateCreated |
1983-5-27
|
|
pubmed:abstractText |
Novel derivatives of the triostin group of antibiotics were prepared by supplementing cultures of the producing organism Streptomyces triostinicus with a variety of aromatic carboxylic acids. Five new antibiotics, each having both the natural quinoxaline chromophores replaced by a substituted ring system, were purified to homogeneity and characterized by high-pressure liquid chromatography and nuclear magnetic resonance. Their antibacterial activities and DNA-binding properties were investigated. Addition of a halogen atom at position 6 of the quinoxaline ring or an amino group at position 3 had little effect on either the biological activity or the DNA-binding characteristics. The bis-3-amino derivative is fluorescent, and its fluorescence is strongly quenched by calf thymus DNA and polydeoxyguanylate-polydeoxycytidylate but not by polydeoxyadenylate-polydeoxythymidylate, suggesting that it binds preferentially to guanosine-cytosine-rich sequences in natural DNA. Binding constants for the bis-6-chloro and bis-3-amino derivatives do not differ greatly from those of unsubstituted triostin A. The analogs having two quinoline chromophores or a chlorine atom in position 7 of the quinoxaline ring display little or no detectable antibacterial activity, in marked contrast to the other congeners. Bis-7-chloro-triostin A binds conspicuously more tightly to polydeoxyadenylate-polydeoxythymidylate than to any other polynucleotide tested.
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6838186-1133418,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6838186-1150534,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6838186-1237323,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6838186-127172,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6838186-4211843,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6838186-4416620,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6838186-4437614,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6838186-5364719,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6838186-5708318,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6838186-6032510,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6838186-687363,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6838186-687364,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6838186-7066328,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6838186-7193050,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6838186-7283970,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6838186-931789,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6838186-985413
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Feb
|
|
pubmed:issn |
0066-4804
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
23
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
221-31
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
|
|
pubmed:year |
1983
|
|
pubmed:articleTitle |
Preparation and DNA-binding properties of substituted triostin antibiotics.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
