Linked Life Data

6836543

Source:http://linkedlifedata.com/resource/pubmed/id/6836543

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1983-5-27
pubmed:abstractText
Lipopolysaccharide (LPS) in concentrations up to 10 micrograms/ml did not induce detectable direct cytotoxicity in human umbilical vein, pulmonary artery, or pulmonary vein endothelial cells. By contrast, significant cytotoxicity was observed in bovine aortic endothelial cells exposed to LPS 0.01 micrograms/ml. Transmission electron microscopy of human umbilical vein cells exposed to LPS 10 micrograms/ml for 4 days revealed no significant ultrastructural abnormalities compared to control cells. Whereas human umbilical vein endothelial cell cytotoxicity was observed when neutrophils were stimulated with phorbol myristate acetate, LPS-stimulated neutrophils did not induce significant cytotoxicity even in the presence of fresh human serum as a complement source. Moreover, human umbilical vein endothelial cell factor VIII-antigen and fibronectin release, angiotensin-converting enzyme activity, and PGI2 release were unaffected by a 24-hour exposure to LPS. Cytotoxicity, however, was produced when human umbilical vein endothelial cells were coincubated with LPS and cycloheximide. The proliferation of human umbilical vein endothelial cells was also inhibited after prolonged, continuous exposure to LPS 10 micrograms/ml. We conclude that LPS with or without complement or neutrophils does not induce significant human endothelial cell lysis or detachment. Moreover, brief exposure to LPS has minimal, direct effect on several functions of human endothelial cells in vitro.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0049-3848
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
29
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
15-26
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1983
pubmed:articleTitle
Effects of lipopolysaccharide on human endothelial cells in culture.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't