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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
1983-5-27
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pubmed:abstractText |
A new medium, norleucine-tyrosine (NT) broth, was developed for rapid identification of Clostridium difficile on the basis of caproic acid and p-cresol production. The NT broth consists of 0.5% Trypticase (BBL Microbiology Systems, Cockeysville, Md.), 0.5% yeast extract (Difco Laboratories, Detroit), 0.2% L-norleucine and 0.2% L-tyrosine (wt/vol; final concentrations), and a mixture of salts. The procedure for demonstrating caproic acid and p-cresol production involves extracting NT broth cultures with ether or chloroform and analyzing the extracts with a gas-liquid chromatograph equipped with a thermal conductivity detector, as is customary for analysis of volatile fatty acids. A total of 120 strains of C. difficile from diverse geographic locations were tested by this procedure, and they all produced caproic acid and p-cresol in NT broth. No other Clostridium species or other microorganisms tested have been found to produce both products in NT broth.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6833488-1275638,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6833488-521486,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6833488-7000811,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6833488-7019263,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6833488-7068826
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/4-cresol,
http://linkedlifedata.com/resource/pubmed/chemical/Cresols,
http://linkedlifedata.com/resource/pubmed/chemical/Hexanoic Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Norleucine,
http://linkedlifedata.com/resource/pubmed/chemical/Tyrosine
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0095-1137
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
17
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
382-5
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading | |
pubmed:year |
1983
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pubmed:articleTitle |
Norleucine-tyrosine broth for rapid identification of Clostridium difficile by gas-liquid chromatography.
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pubmed:publicationType |
Journal Article
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