Linked Life Data

6830019

Source:http://linkedlifedata.com/resource/pubmed/id/6830019

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1983-4-21
pubmed:abstractText
A procedure for isolation of the turkey heterophil was developed. The procedure involves preliminary lysis of erythrocytes, using ammonium chloride and centrifugation of the leukocytes over a discontinuous gradient of 18% and 24% Ficoll. A minimum of 30% of the heterophils was consistently recovered from whole blood by application of this procedure. Spontaneous and chemotactic migratory responses of the isolated heterophils were assessed, using the migration under agarose technique. Supplementation of the agarose with 10% heat-inactivated turkey plasma and extension of the incubation period to 3 hours at 41 C in a 5% CO2-humidified atmosphere were required for optimal chemotaxis in response to zymosan-activated chicken serum. The heterophils did not respond chemotactically to the oligopeptide attractant, N-formyl-methionyl-leucyl-phenylalanine, and did not specifically bind a radiolabeled form of this ligand.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0002-9645
pubmed:author
pubmed:issnType
Print
pubmed:volume
44
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
288-92
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1983
pubmed:articleTitle
Isolation of the turkey heterophil and measurement of its migratory functions under agarose.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't