Linked Life Data

6829763

Source:http://linkedlifedata.com/resource/pubmed/id/6829763

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1983-4-15
pubmed:abstractText
Accurate evaluation of the unavoidable liquid junction potentials (LJP) involved whenever bath (B) and perfusate (P) solutions differ in ionic composition is of fundamental importance in the determination of proximal convoluted tubule (PCT) transepithelial potential, psi T, and its active and diffusion components, psi A and psi D. Therefore, a precise method of measuring these LJP, using Ag/AgCl electrodes, has been developed and successfully tested. The measured LJP, psi B-P, between artificial bath serum containing 6 g/100 ml albumin and ultrafiltrate-like perfusate is +2.6 mV. The average measured potential difference (PD) in rabbit PCT with these solutions was -3.0 mV, psi T thus being only -0.4 mV. Three maneuvers known to abolish psi A (hypothermia, 10(-4) ouabain, or K-free bath) brought the PD close to the reference potential, indicating that psi A congruent to PD and that psi D congruent to psi B-P. From dilution potentials, after correction for LJP (4.3 in the absence and 6.2 mV in the presence of proteins), PNa/PCl was calculated to be 0.9 for midcortical and 1.1 for juxtamedullary PCT in the absence of proteins and slightly lower in their presence. These values of LJP obtained with Ag/AgCl electrodes are between 30 and 100% larger than the ones measured using a saturated KCl bridge or the ones in previous studies, indicating that in these studies psi T as well as PNa/PCl has been overestimated.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0002-9513
pubmed:author
pubmed:issnType
Print
pubmed:volume
244
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
F304-19
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1983
pubmed:articleTitle
Liquid junctions and isolated proximal tubule transepithelial potentials.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't