Linked Life Data

6827807

Source:http://linkedlifedata.com/resource/pubmed/id/6827807

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1983-4-21
pubmed:abstractText
Lipopolysaccharide (LPS) produced time- and dose-dependent bovine endothelial cell injury in vitro that was manifested initially by cell detachment from culture substrate with subsequent cell lysis. Bovine endothelial cell injury was observed with LPS derived from Salmonella minnesota R595, a LPS comprised only of lipid A and a trisaccharide core, as well as intact LPS preparations derived Escherichia coli and S. typhosa. LPS-mediated bovine endothelial cell detachment was prevented by incubation at 4 degrees C but was not prevented by indomethacin, lidocaine, chlorpromazine or trifluoperazine, methylprednisolone or p-bromophenacyl bromide, protease inhibitors, and catalase or superoxide dismutase. Of note, LPS-mediated injury was markedly enhanced by cycloheximide. Although augmented by serum, LPS-mediated bovine endothelial cell detachment was observed in C8-deficient serum and also in serum-free medium at higher LPS concentrations. Bovine aortic, pulmonary artery, mesenteric artery, and mesenteric vein endothelial cells were all sensitive to LPS at a concentration of 1 microgram/ml. In contrast, bovine aortic smooth muscle, human umbilical vein, goat aortic, and canine vena cava endothelial cells were unaffected by LPS at a concentration of 100 micrograms/ml. We conclude that the lipid A moiety of LPS mediates direct, complement-independent endothelial cell cytotoxicity and that this injury is not prevented by inhibitors of protein and prostaglandin synthesis, oxygen radical production, protease and phospholipase activity, and cytoskeletal function. Importantly, this direct LPS-mediated cytotoxic effect is dependent on the species from which the endothelial cells are derived.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0023-6837
pubmed:author
pubmed:issnType
Print
pubmed:volume
48
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
269-74
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1983
pubmed:articleTitle
Lipopolysaccharide-mediated bovine endothelial cell injury in vitro.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't