Linked Life Data

6827478

Source:http://linkedlifedata.com/resource/pubmed/id/6827478

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1983-4-7
pubmed:abstractText
NADH fluorescence (366 leads to 450 nm) was measured with a large tipped light guide from the surface of rat liver perfused with hemoglobin-free medium without recirculation. A good correlation (r = 0.93) was found between the increase in NADH fluorescence and the rate of oxidation of infused acetaldehyde (0.1-4.0 mM). By using microlight guides placed on either periportal or pericentral areas of the liver, it was found that infusion of acetaldehyde increased NADH fluorescence first in periportal regions and then in pericentral areas. During retrograde perfusions, NADH fluorescence in pericentral areas increased before portal areas. By using the correlation between the increase in NADH fluorescence and the rate of acetaldehyde uptake established with the large light guide, rates of acetaldehyde uptake in pericentral and periportal areas were estimated from the microlight guide measurement of the periportal and pericentral NADH fluorescence increments caused by acetaldehyde infusions. Half-maximal acetaldehyde uptake was observed at about 0.8 mM in both regions. Maximal rates of acetaldehyde uptake were 212 and 197 mumol/g/hr in periportal and pericentral regions, respectively. The results indicate that rates of aldehyde dehydrogenase-dependent acetaldehyde oxidation are similar in periportal and pericentral regions of the liver lobule.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0022-3565
pubmed:author
pubmed:issnType
Print
pubmed:volume
224
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
538-42
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1983
pubmed:articleTitle
Aldehyde dehydrogenase-dependent acetaldehyde metabolism in periportal and pericentral regions of the perfused rat liver.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.