Linked Life Data

6822573

Source:http://linkedlifedata.com/resource/pubmed/id/6822573

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1983-3-24
pubmed:abstractText
The transport of D-aspartate has been studied in an epithelial cell line from a pig kidney. The amino acid is accumulated by LLC-PK1 cells without evidence of metabolism. The accumulation against a concentration gradient occurs through a mechanism with several features of a carrier-mediated process. The influx may be accounted for by a saturable Na+-dependent and nonsaturable Na+-independent process. The presence of Na+ in the incubation medium increases Vmax without affecting Km. A number of differences in the apparent affinities and specifities allows one to differentiate between this and the acidic amino acid transport system from other tissues. Polarized uptake from either side of the monolayers indicates that the acidic amino acid transport system is preferentially located in the apical membrane of the cultured renal cells. The apical localization of this system clearly contrasts with the basolateral localization of the other three neutral amino acid transport systems reported previously, indicating a high degree of cell polarization. The present study shows a close similarity between the Na+-dependent acidic amino acid transport system in LLC-PK1 cells and the system present in the apical membrane of the proximal tubular cells.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
25
pubmed:volume
258
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2543-7
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1983
pubmed:articleTitle
Polarized amino acid transport by an epithelial cell line of renal origin (LLC-PK1). The apical systems.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.