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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
7
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pubmed:dateCreated |
1982-12-21
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pubmed:abstractText |
Hemolytic activity of the culture supernatant of Leptospira interrogans serovar canicola strain Moulton grown in protein-free medium was demonstrated. The activity began to appear in the late logarithmic phase of growth of the organism and reached a plateau after 2 weeks of cultivation. It was inactivated by the addition of dipotassium ethylenediaminetetraacetate, but was effectively restored by Mg2+. Hemolysis by the culture supernatant was stimulated by "hot-cold" incubation. Sleep erythrocytes treated with the culture supernatant of the organism were transformed into spherocytes, in which invagination was observed. A hemolysis inhibitor in rabbit serum was found to be in the chloroform-methanol soluble fraction of the serum. The hemolysin of Leptospira may be phospholipase C.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:issn |
0385-5600
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
26
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
547-56
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:6813653-Animals,
pubmed-meshheading:6813653-Culture Media,
pubmed-meshheading:6813653-Edetic Acid,
pubmed-meshheading:6813653-Erythrocytes,
pubmed-meshheading:6813653-Hemolysin Proteins,
pubmed-meshheading:6813653-Hot Temperature,
pubmed-meshheading:6813653-Hydrogen-Ion Concentration,
pubmed-meshheading:6813653-Leptospira interrogans,
pubmed-meshheading:6813653-Rabbits,
pubmed-meshheading:6813653-Sheep
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pubmed:year |
1982
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pubmed:articleTitle |
Hemolytic activity of Leptospira interrogans serovar canicola cultured in protein-free medium.
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pubmed:publicationType |
Journal Article
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