Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1982-12-2
pubmed:abstractText
N-Methyl-N'-nitro-N-nitrosoguanidine (MNNG)-induced repair (unscheduled) DNA synthesis was studied in intact HeLa cells as well as in the cell lysate. The incorporation of dTTP[methyl-3H] in vitro by the cell lysate was dependent on the dose of MNNG, and as linear for 2 min, reaching the maximum at 4 min. The optimum conditions were the same as for UV-induced in vitro repair synthesis. The presence of hydroxyurea and arabinofuranosyl cytosine during in vivo incubation with MNNG was necessary for enhancement of in vitro DNA synthesis. This MNNG-induced in vitro DNA synthesis was inhibited by aphidicolin, but not by 2',3'-dideoxythymidine-5'-triphosphate. It thus appears that DNA polymerase alpha participates in MNNG-induced unscheduled DNA synthesis in vitro.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0016-450X
pubmed:author
pubmed:issnType
Print
pubmed:volume
73
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
63-9
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:year
1982
pubmed:articleTitle
Unscheduled DNA synthesis induced by N-methyl-N'-nitro-N-nitrosoguanidine in Hela cells.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't