Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1982-10-29
|
| pubmed:abstractText |
Structural and immunochemical studies have been performed on the O-specific antigens of two serologically related types of Pseudomonas aeruginosa O:2a,b and O:2a,c (Lanyi's classification). The O-specific polysaccharide chains of the two serotypes were shown to be acidic polysaccharides composed of repeating trisaccharide units consisting of L-rhamnose, N-acetyl-D-quinovosamine and N-acetyl-L-galactosaminuronic acid residues. Based on 1H and 13C nuclear magnetic resonance spectroscopy, data of methylation analysis and selective solvolysis with hydrogen fluoride, the repeating unit of the O:2a,c O-specific polysaccharide was assigned the following structure: leads to 4)LGalNAcA(alpha1 leads to 3)DQuiNAc(alpha1 leads to 3) LRha(alpha1, where GalNAcA = N-acetylgalactosaminuronic acid, QuiNAc = N-acetylquinovosamine and Rha = rhamnose. The O:2a,b O-specific polysaccharide had the same structure of the trisaccharide repeating unit but was distinguished by the presence of O-acetyl groups on 70-80% of the rhamnose residues in position 2. The O-acetyl groups were located both by methylation with methyltrifluoromethane sulphonate and by comparison of the 13C nuclear magnetic resonance spectra of the native and O-deacetylated polysaccharides. Serological studies revealed the determinant role of the O-acetyl groups in the O-antigen O:2a,b and suggested the O-factor 2b to be related to the 2-O-acetyl-L-rhamnose residue, whereas the O-factor 2c was probably determined by the nonacetylated rhamnose residue. The dominant moiety of the determinant 2a common to the two antigens was obviously presented by the N-acetyl-L-galactosaminuronic acid residue.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jun
|
| pubmed:issn |
0014-2956
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
15
|
| pubmed:volume |
125
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
221-7
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading |
pubmed-meshheading:6809463-Acetylation,
pubmed-meshheading:6809463-Chemical Phenomena,
pubmed-meshheading:6809463-Chemistry,
pubmed-meshheading:6809463-Hydrolysis,
pubmed-meshheading:6809463-Lipopolysaccharides,
pubmed-meshheading:6809463-Magnetic Resonance Spectroscopy,
pubmed-meshheading:6809463-Pseudomonas aeruginosa
|
| pubmed:year |
1982
|
| pubmed:articleTitle |
Somatic antigens of Pseudomonas aeruginosa. The structure of the O-specific polysaccharide chains of Ps.aeruginosa O:2 (Lanyi) lipopolysaccharides.
|
| pubmed:publicationType |
Journal Article
|